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Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells BJMBR
Xue,Meilan; Ge,Yinlin; Zhang,Jinyu; Wang,Qing; Hou,Lin.
Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorella vulgaris; CvFad3 gene; Fatty acid desaturase; Recombinant expression vector; Fatty acid composition; Breast cancer cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200006
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Hematological and biochemical responses of pirarucu (Arapaima gigas, Arapaimidae) fed with diets containing a glucomannan product derived from yeast and algae Acta Amazonica
HOSHINO,Maria Danielle Figueiredo Guimarães; MARINHO,Renata das Graças Barbosa; PEREIRA,Diogle Farias; YOSHIOKA,Eliane Tie Oba; TAVARES-DIAS,Marcos; OZORIO,Rodrigo Otavio de Almeida; RODRIGUEZ,Anselmo Fortunato Ruiz; RIBEIRO,Ricardo Amaral; FARIA,Fernando Sérgio Escócio Drummond Viana de.
ABSTRACT The hematological and biochemical responses of pirarucu fingerlings (Arapaima gigas) fed with diets containing different concentrations of a glucomannan product derived from yeast and algae were evaluated in order to ascertain the effect of these diets on fish physiology. Four treatments were conducted, with three replications, with 12 fish in each tank. The product evaluated (MycosorbA+(r)) was incorporated into the commercial diet, at four concentrations: 0, 1, 2 and 4 g.kg-1, called M0%, M0.1%, M0.2% and M0.4%, respectively. After 45 days of feeding, blood samples from six fish in each replicate were collected to perform the analyses. Their weight and length were determined to calculate the condition factor and weight gain, but no differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorella vulgaris; Saccharomyces cerevisiae; Leukocytes; Albumin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000200087
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INMOVILIZACIÓN DE LAS MICROALGAS Scenedesmus ovalternus (Scenedesmaceae) Y Chlorella vulgaris (Chlorellaceae) EN ESFERAS DE ALGINATO DE CALCIO Acta biol.Colomb.
FORERO-CUJIÑO,Mario Andrés; MONTENGRO RUIZ,Luis Carlos; PINILLA-AGUDELO,Gabriel Antonio; MELGAREJO-MUÑOZ,Luz Marina.
En este trabajo se describe la técnica de inmovilización de microalgas en esferas de alginato de calcio. Se emplearon las especies Scenedesmus ovalternus y Chlorella vulgaris, se determinó la estabilidad de las esferas, la cinética de crecimiento y la concentración de las microalgas en el interior de las esferas. Chlorella vulgaris alcanzó mayores densidades poblacionales y tasas de crecimiento más altas cuando se inmovilizó en concentraciones del 10 % v/v con el alginato (1,31*10(6) cél/ml). Para Scenedesmus ovalternus se observó una mayor densidad poblacional y una mayor tasa de crecimiento cuando se inmovilizó en concentraciones del 20 % v/v (7,06*10(5) cél/ml). Estos resultados son útiles para aplicaciones prácticas de las algas encapsuladas, tales...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alginato de calcio; Chlorella vulgaris; Microalgas inmovilizadas; Técnica de inmovilización; Scenedesmus ovalternus.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200012
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Microalgae population dynamics in photobioreactors with secondary sewage effluent as culture medium BJM
Marchello,Adriano E.; Lombardi,Ana T.; Dellamano-Oliveira,Maria José; Souza,Clovis W.O. de.
Nitrogen and phosphorus present in sewage can be used for microalgae growth, possibiliting cost reduction in the production of microalgae at the same time that it decreases the eutrophication potential of the effluent. This research aimed at monitoring the native community of microalgae and coliform bacteria in a secondary effluent from anaerobic municipal sewage treatment. Two treatments (aerated and non-aerated) were performed to grow microalgae under semi-controlled conditions in semi-closed photobioreactors in a greenhouse. The results showed no significant pH and coliforms (total and Escherichia coli) variation between treatments. Nutrient concentrations were reduced supporting microalgae growth up to 107 cells.mL−1independent of aeration. Exponential...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Effluent treatment; Coliforms; Escherichia coli; Chlorella vulgaris.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100075
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Producción de biodiésel a partir de microalgas y una cianobacteria cultivadas en diferentes calidades de agua Agrociencia
Sacristan-de Alva,Manuel; Luna-Pabello,Víctor M.; Cadena-Martínez,Erasmo; Alva-Martínez,Alejandro F..
La producción de biodiésel, a partir de microorganismos fotosintéticos, es un tema de creciente investigación. No obstante, las implicaciones de la remoción de nutrientes y capacidad de acumulación de lípidos se desconocen cuando los microorganismos se cultivan en aguas residuales municipales respecto de su cultivo en un medio enriquecido. En el presente estudio se evaluó el potencial de dos especies de microalgas, Chlorella vulgaris y Scenedesmus acutus, y de una cianobacteria, Arthrospira maxima, para remover nutrientes y acumular lípidos, útiles para producir biodiésel, al cultivarse en dos calidades de agua residual, cruda (ARC) y tratada (ART), comparadas con un medio enriquecido (AE) con fertilizante comercial. Los cultivos se realizaron en volúmenes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aguas residuales; Arthrospira maxima; Biodié-sel; Chlorella vulgaris; Remoción de nutrientes; Scenedesmus acutus.
Ano: 2014 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000300003
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THE EFFECT OF CHLORELLA VULGARIS MICROALGAE ON SOME CHARECTERISTICS OF STORAGE TIME OF DATE FRUIT (phoenix dactylifera) CIGR Journal
Hashemi, Ali; Hesami, Abdoalali; Ghasemi, Abdoalhosein.
The unsuitable storage conditions and the type of date pack are one of the most important problems in the palm industry. In this research, two date cultivars Kabkab and Khasoui, microalgae with the name of Chlorella vulgaris at four levels , 0, 25, 50 and 100% and time of storage in three levels 1, 2 and three mounts (90 days) were carried out  as factorial experiment in a completely randomized design with three replications. Dry weight, moisture content, pH and rate of decay of fruits of both cultivars were measured in every 30 days. Based on the results, the effect of microalgae and different months sampling for all traits were significant at (p ≤ 0.01) level. The effects of Chlorella vulgaris at 25% level was higher than the other concentrations on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Storage time; Dates; Chlorella vulgaris; Algae Extract..
Ano: 2022 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/7285
Registros recuperados: 6
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